Lab Members


Koutsioulis D, Wang E, Tzanodaskalaki M, Nikiforaki D, Deli A, Feller G, Heikinheimo P, Bouriotis V. Directed evolution on the cold adapted properties of TAB5 alkaline phosphatase.
Protein Eng Des Sel. 2008 May;21(5):319-27.
Michaux C, Massant J, Kerff F, Frère JM, Docquier JD, Vandenberghe I, Samyn B, Pierrard A, Feller G, Charlier P, Van Beeumen J, Wouters J. Crystal structure of a cold-adapted class C beta-lactamase.
FEBS J. 2008 Apr;275(8):1687-97. Epub 2008 Feb 29.
Marx JC, Poncin J, Simorre JP, Ramteke PW, Feller G. The noncatalytic triad of alpha-amylases: a novel structural motif involved in conformational stability.
Proteins. 2008 Feb 1;70(2):320-8.


Sonan GK, Receveur-Brechot V, Duez C, Aghajari N, Czjzek M, Haser R, Gerday C. The linker region plays a key role in the adaptation to cold of the cellulase from an Antarctic bacterium.
Biochem J. 2007 Oct 15;407(2):293-302.
Collins T, Roulling F, Piette F, Marx JC, Feller G, Gerday C and D'Amico S. Fundamentals of Cold-Adapted Enzymes.
In Margesin R, Schinner F, Gerday C and Marx JC. (Eds), Psychrophiles: from biodiversity to biotechnology. Springer-Verlag, In press.
Enzymes Tables
Collins T, D'Amico S, Marx J, Feller G and Gerday C. Cold-Adapted Enzymes. In Gerday, C. and Glansdorff, N. (eds.), Physiology and biochemistry of extremophiles. ASM Press, Washington, D.C., pp. 165-179.
Gerday C, D'Amico S, Collins T, Marx JC, Feller G and Sonan G. Adaptation strategies and uses of cold adapted enzymes in biotechnological processes. In JAMSTEC, E.R.C. (ed.) , Proceedings of the International Symposium on Extremophiles and their Applications 2005. Japan Science and Technology Information Aggregator, Electronic (J-STAGE) , Tokyo , Japan , pp. 66-72.
Collins T, Hoyoux A, Van Petegem F, De Vos D, Van Beeumen J, Stals I, Nerinckx W, Claeyssens MJGTD, Arnaut F, Gerday C and Feller G. A novel family 8 psychrophilic xylanase: fundamentals and application. In JAMSTEC, E.R.C. (ed.), Proceedings of the International Symposium on Extremophiles and their Applications 2005. Japan Science and Technology Information Aggregator, Electronic (J-STAGE), Tokyo, Japan, pp. 73-79.
Srimathi S, Jayaraman G, Feller G, Danielsson B, Narayanan PR. Intrinsic halotolerance of the psychrophilic alpha-amylase from Pseudoalteromonas haloplanktis. Extremophiles. 2007 Feb 20; [Epub ahead of print]. abstract
Feller G. Life at low temperatures: is disorder the driving force?
Extremophiles. 2007 Mar;11(2):211-6.
Marx JC, Collins T, D'Amico S, Feller G, Gerday C. Cold-Adapted Enzymes from Marine Antarctic Microorganisms.
Mar Biotechnol (NY). 2007 May-Jun;9(3):293-304.


Siddiqui KS, Poljak A, Guilhaus M, De Francisci D, Curmi PM, Feller G, D'Amico S, Gerday C, Uversky VN, Cavicchioli R. Role of lysine versus arginine in enzyme cold-adaptation: Modifying lysine to homo-arginine stabilizes the cold-adapted alpha-amylase from Pseudoalteramonas haloplanktis.
Proteins. 2006 Aug 1;64(2):486-501.
De Vos D, Collins T, Nerinckx W, Savvides SN, Claeyssens M, Gerday C, Feller G, Van Beeumen J. Oligosaccharide binding in family 8 glycosidases: crystal structures of active-site mutants of the beta-1,4-xylanase pXyl from Pseudoaltermonas haloplanktis TAH3a in complex with substrate and product.
Biochemistry. 2006 Apr 18;45(15):4797-807.
D'Amico S, Collins T, Marx JC, Feller G, Gerday C.
Psychrophilic microorganisms: challenges for life.
EMBO Rep. 2006 Apr;7(4):385-9. 
D'Amico S, Sohier JS, Feller G. Kinetics and energetics of ligand binding determined by microcalorimetry: insights into active site mobility in a psychrophilic alpha-amylase.
J Mol Biol. 2006 May 19;358(5):1296-304. 
Collins T, D'Amico S, Georlette D, Marx JC, Huston AL, Feller G. A nondetergent sulfobetaine prevents protein aggregation in microcalorimetric studies.
Anal Biochem. 2006 May 15;352(2):299-301. 
Collins T, Hoyoux A, Dutron A, Georis J, Genot B, Dauvrin T, Arnaut F, Gerday C, Feller, G. Use of glycoside hydrolase family 8 xylanases in baking.
J Cereal Science. 2006 ;43:79–84. 


Georis J, Dauvrin T, Hoyoux, A, Collins, T, Feller, G. Novel Xylanases and their use.
2005; Patent Application EP 1 574 567 A1.
Collins T, De Vos D, Hoyoux A, Savvides SN, Gerday C, Van Beeumen J, Feller G. Study of the Active Site Residues of a Glycoside Hydrolase Family 8 Xylanase.
J Mol Biol. 2005 Nov 25;354(2):425-35.
Zecchinon L, Oriol A, Netzel U, Svennberg J, Gerardin-Otthiers N, Feller G. Stability domains, substrate-induced conformational changes and hinge-bending motions in a psychrophilic phosphoglycerate kinase: A microcalorimetric study.
J Biol Chem. 2005 Dec 16;280(50):41307-14.
Medigue C, Krin E, Pascal G, Barbe V, Bernsel A, Bertin PN, Cheung F, Cruveiller S, D'Amico S, Duilio A, Fang G, Feller G, Ho C, Mangenot S, Marino G, Nilsson J, Parrilli E, Rocha EP, Rouy Z, Sekowska A, Tutino ML, Vallenet D, von Heijne G, Danchin A. Coping with cold: the genome of the versatile marine Antarctica bacterium Pseudoalteromonas haloplanktis TAC125.
Genome Res. 2005 Oct;15(10):1325-35.
Siddiqui KS, Poljak A, Guilhaus M, Feller G, D'Amico S, Gerday C, Cavicchioli R. Role of Disulfide Bridges in the Activity and Stability of a Cold-Active {alpha}-Amylase.
J Bacteriol. 2005 Sep;187(17):6206-12.
Siddiqui KS, Feller G, D'Amico S, Gerday C, Giaquinto L, Cavicchioli R. The Active Site Is the Least Stable Structure in the Unfolding Pathway of a Multidomain Cold-Adapted {alpha}-Amylase.
J Bacteriol. 2005 Sep;187(17):6197-205.
Violot S, Aghajari N, Czjzek M, Feller G, Sonan GK, Gouet P, Gerday C, Haser R, Receveur-Brechot V. Structure of a full length psychrophilic cellulase from Pseudoalteromonas haloplanktis revealed by X-ray diffraction and small angle X-ray scattering.
J Mol Biol. 2005 May 20;348(5):1211-24. Epub 2005 Mar 25.
Collins T, Gerday Ch, Feller G. Xylanases, xylanase families and extremophilic xylanases.
FEMS Microbiol Rev. 2005 Jan;29(1):3-23. 


Garsoux G, Lamotte J, Gerday C, Feller G. Kinetic and structural optimization to catalysis at low temperatures in a psychrophilic cellulase from the Antarctic bacterium Pseudoalteromonas haloplanktis.
Biochem J. 2004 Dec 1;384(Pt 2):247-53.
Hoyoux A, Blaise V, Collins T, D'Amico S, Gratia E, Huston AL, Marx JC, Sonan G, Zeng Y, Feller G, Gerday C. Extreme catalysts from low-temperature environments.
J. Biosci. Bioeng. 2004;98(5):317-30.
Marx JC, Blaise V, Collins T, D'Amico S, Delille D, Gratia E, Hoyoux A, Huston AL, Sonan G, Feller G, Gerday C. A perspective on cold enzymes: current knowledge and frequently asked questions.
Cell Mol Biol (Noisy-le-grand). 2004 Jul;50(5):643-55.
D'Amico S, Georlette D, Collins T, Feller G, Gerday C. Microcalorimetry as applied to psychrophilic enzymes.
In: Biocalorimetry 2 : Applications of calorimetry in the biological sciences, Eds J.E. Ladbury and M.L. Doyle, John Wiley & Sons, Chichester, UK.
Dutron A, Georis j, Genot B, Dauvrin T, Collins T, Hoyoux A, Feller G. Use of family 8 enzymes with xylanolytic activity in baking.
In: World Intellectual Property Organization, PCT, WO 2004/023879 A1.
Georlette D, Blaise V, Bouillenne F, Damien B, Thorbjarnardottir SH, Depiereux E, Gerday C, Uversky VN, Feller G. Adenylation-dependent conformation and unfolding pathways of the NAD+-dependent DNA ligase from the thermophile Thermus scotoductus.
Biophys J. 2004 Feb;86(2):1089-104. 
Georlette D, Blaise V, Collins T, D'Amico S, Gratia E, Hoyoux A, Marx JC, Sonan G, Feller G, Gerday C. Some like it cold: biocatalysis at low temperatures.
FEMS Microbiol Rev. 2004 Feb;28(1):25-42.
Da Lage JL, Feller G, Janecek S. Horizontal gene transfer from Eukarya to bacteria and domain shuffling: the alpha-amylase model.
Cell Mol Life Sci. 2004 Jan;61(1):97-109.


Feller G, Gerday C.
Psychrophylic enzymes: hot topics in cold adaptation.
Nature Reviews Microbiology 2003 Dec; 1:200-208. 
Georlette D, ;Blaise V, Dohmen C, Bouillenne F, Damien B, Depiereux E, Gerday C, Uversky V, Feller G. Cofactor binding modulates the conformational stabilities and unfolding patterns of NAD+-dependent DNA ligases from Escherichia coli and Thermus scotoductus.
J Biol Chem. 2003 Dec 12;278(50):49945-53.
Xu Y, Feller G, Gerday C, Glansdorff N. Moritella cold-active dihydrofolate reductase: are there natural limits to optimization of catalytic efficiency at low temperature?
J Bacteriol. 2003 Sep;185(18):5519-26.  
D'Amico S, Gerday C, Feller G. Temperature Adaptation of Proteins: Engineering Mesophilic-like Activity and Stability in a Cold-adapted alpha-Amylase.
J Mol Biol. 2003 Oct 3;332(5):981-8. 
Feller G. Molecular adaptations to cold in psychrophilic enzymes.
Cell Mol Life Sci. 2003 Apr;60(4):648-62. Review. 
Mavromatis K, Feller G, Kokkinidis M, Bouriotis V. Cold adaptation of a psychrophilic chitinase: a mutagenesis study.
Protein Eng. 2003 Jul;16(7):497-503.
Xu Y, Feller G, Gerday C, Glansdorff N. Metabolic enzymes from psychrophilic bacteria: challenge of adaptation to low temperatures in ornithine carbamoyltransferase from Moritella abyssi.
J Bacteriol. 2003 Apr;185(7):2161-8. 
Aghajari N, Van Petegem F, Villeret V, Chessa JP, Gerday C, Haser R, Van Beeumen J. Crystal structures of a psychrophilic metalloprotease reveal new insights into catalysis by cold-adapted proteases.
Proteins. 2003 Mar 1;50(4):636-47.
Claverie P, Vigano C, Ruysschaert JM, Gerday C, Feller G. The precursor of a psychrophilic alpha-amylase: structural characterization and insights into cold adaptation.
Biochim Biophys Acta. 2003 Jul 30;1649(2):119-22. 
Violot S, Haser R, Sonan G, Georlette D, Feller G, Aghajari N. Expression, purification, crystallization and preliminary X-ray crystallographic studies of a psychrophilic cellulase from Pseudoalteromonas haloplanktis.
Acta Crystallogr D Biol Crystallogr. 2003 Jul;59(Pt 7):1256-8. 
Georlette D, Damien B, Blaise V, Depiereux E, Uversky VN, Gerday C, Feller G.
Structural and functional adaptations to extreme temperatures in psychrophilic, mesophilic and thermophilic DNA ligases.
J Biol Chem. 2003 Sep 26;278(39):37015-23. 
Collins T, Meuwis MA, Gerday C, Feller G. Activity, stability and flexibility in glycosidases adapted to extreme thermal environments.
J Mol Biol. 2003 Apr 25;328(2):419-28. 
D'Amico S, Marx JC, Gerday C, Feller G. Activity-stability relationships in extremophilic enzymes.
J Biol Chem. 2003 Mar 7;278(10):7891-6. Epub 2003 Jan 02. 
Van Petegem F, Collins T, Meuwis MA, Gerday C, Feller G, Van Beeumen J. The structure of a cold-adapted family 8 xylanase at 1.3A resolution, structural adaptations to cold and investigation of the active site.
J Biol Chem. 2003 Feb 28;278(9):7531-9. Epub 2002 Dec 09. 

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